As part of the summer internship at Edge Hill University, I’ve recently gotten out to do some sampling! Related to the topic of the internship, “Does meadow restoration conserve genetic variation”, this sampling trip was a test run for a larger project. A recent Edge Hill graduate, Heather Wickson, and I took a trip over to Wigan and met the Lancashire Wildlife Trust at this branch. They’ve an Edge Hill friend and graduate, Mark Champion, working there and also a current student on a work placement. The team over at the Wigan office, as well as Heather and I, were to help Elizabeth Sullivan on this test run. If we could get the kinks ironed out and prove that this method can work, then she hopes the project can be rolled out over a wider area, having people from other areas collected specimens for genetic analysis.
Setting off for sites such as Wigan Flashes Local Nature Reserve (LNR) and Low Hall LNR to sample Plantago lanceolata (ribwort plantain) and Lotus corniculatus (bird’s-foot trefoil). These areas were teeming with life, plenty of butterflies, damselflies and dragonflies in the air, no doubt a result of the extreme heat we’ve been experiencing for the past few weeks. I managed to get a few good snaps of some butterflies around the area – particularly the common blue. Although I didn’t manage to grab a picture of the dragonflies out that day, I did snap a good one on the first year biology residential field trip to Cyprus.
Since collecting these samples from meadows in the Wigan area, Heather and I have been in the lab, processing samples she and another recent Edge Hill graduate, Katherine Judson, collected a few weeks ago. These samples were of Rhinanthus minor (yellow rattle) and were collected from roadside verges down in Worcestershire. These roadside collections help build up the bigger picture, filling in the gaps of connectivity in meadows as part of Elizabeth’s work.
Currently, Heather and I are extracting DNA from these yellow rattle samples, amplifying them with fluorescently tagged microsatellite markers, and will soon be sequencing them ready for fragment analysis. After sequencing, I hope to help Elizabeth with the analysis and perhaps present a poster on the findings at the upcoming Annual Biology/Geography Postgraduate Research Forum!